Company description
Surface Plasmon Resonance imaging (SPRi), namely surface plasmon resonance microscopy (SPRM), is a real-time, label-free, and high-throughput technique which is used to study biomolecular interactions based on detecting the refractive index changes resulting from molecular binding. More specifically, SPRi has been developed to examine affinity between biomolecules, screen biomarkers and detect biopsy specimens.
Surface plasmon resonance imaging (SPRi) service
Basic principles:
Surface Plasmon Resonance (SPR) is an optical detection process that occurs when a polarized light hits a prism covered by a thin planar metal (typically gold or silver) layer. At certain angles of incidence, a portion of the light energy couples through the gold coating and creates a surface plasmon wave at the sample and gold surface interface. The angle of incident light required to sustain the surface plasmon wave is very sensitive to changes in refractive index at the surface (due to mass change), and it is these changes that are used to monitor the association and dissociation of biomolecules.
