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Common peptide synthesis strategy is solid phase synthesis, which sequence generally synthesized from the N-terminus (amino terminus) to the C-terminus (carboxy terminus). In the past, peptide synthesis is carried out in solution so it is called liquid phase synthesis. Since Merrifield developed the solid phase synthesis method of peptide in 1963, it has been improved and perfected continuously. Today, the solid phase synthesis method has become a kind of common technique in the synthesis of peptides and proteins, showing the advantages unmatched by classical liquid phase synthesis, thus reducing the difficulty of purification of each step of the product greatly.
